Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase

Cinzia Cinesi; Lorène Aeschbach; Bin Yang; Vincent Dion

doi:10.1038/ncomms13272

Nature Communications (Nov 2016)

Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase

Cinzia Cinesi,
Lorène Aeschbach,
Bin Yang,
Vincent Dion

Affiliations

Cinzia Cinesi: Center for Integrative Genomics, University of Lausanne
Lorène Aeschbach: Center for Integrative Genomics, University of Lausanne
Bin Yang: Center for Integrative Genomics, University of Lausanne
Vincent Dion: Center for Integrative Genomics, University of Lausanne

DOI: https://doi.org/10.1038/ncomms13272
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 10

Abstract

Read online

The expansion of trinucleotide repeats has been linked to several neurodegenerative disorders. Here, the authors show that the CRISPR-Cas9 nuclease induces both expansions and contractions of the repeat region, whereas the nickase leads predominantly to contractions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal