Frontiers in Immunology (Apr 2020)

Salmonella Enteritidis Effector AvrA Suppresses Autophagy by Reducing Beclin-1 Protein

  • Yang Jiao,
  • Yong-guo Zhang,
  • Zhijie Lin,
  • Rong Lu,
  • Yinglin Xia,
  • Chuang Meng,
  • Zhimin Pan,
  • Xiulong Xu,
  • Xinan Jiao,
  • Jun Sun

DOI
https://doi.org/10.3389/fimmu.2020.00686
Journal volume & issue
Vol. 11

Abstract

Read online

Autophagy is a cellular process to clear pathogens. Salmonella enterica serovar Enteritidis (S.E) has emerged as one of the most important food-borne pathogens. However, major studies still focus on Salmonella enterica serovar Typhimurium. Here, we reported that AvrA, a S. Enteritidis effector, inhibited autophagy to promote bacterial survival in the host. We found that AvrA regulates the conversion of LC3 I into LC3 II and the enrichment of lysosomes. Beclin-1, a key molecular regulator of autophagy, was decreased after AvrA expressed strain colonization. In S.E-AvrA−-infected cells, we found the increases of protein levels of p-JNK and p-c-Jun and the transcription level of AP-1. AvrA-reduction of Beclin-1 protein expression is through the JNK pathway. The JNK inhibitor abolished the AvrA-reduced Beclin-1 protein expression. Moreover, we identified that the AvrA mutation C186A abolished its regulation of Beclin-1 expression. In addition AvrA protein was found interacted with Beclin-1. In organoids and infected mice, we explored the physiologically related effects and mechanism of AvrA in reducing Beclin-1 through the JNK pathway, thus attenuating autophagic responses. This finding not only indicates an important role of S. Enteritidis effector in reducing host protein as a strategy to suppress autophagy, but also suggests manipulating autophagy as a new strategy to treat infectious diseases.

Keywords