mSystems (Aug 2021)
Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies
- Christoph Ammer-Herrmenau,
- Nina Pfisterer,
- Tim van den Berg,
- Ivana Gavrilova,
- Ahmad Amanzada,
- Shiv K. Singh,
- Alaa Khalil,
- Rohia Alili,
- Eugeni Belda,
- Karine Clement,
- Ahmed Abd El Wahed,
- ElSagad Eltayeb Gady,
- Martin Haubrock,
- Tim Beißbarth,
- Volker Ellenrieder,
- Albrecht Neesse
Affiliations
- Christoph Ammer-Herrmenau
- Department of Gastroenterology, Gastrointestinal Oncology and Endocrinology, University Medicine Goettingen, Goettingen, Germany
- Nina Pfisterer
- Department of Gastroenterology, Gastrointestinal Oncology and Endocrinology, University Medicine Goettingen, Goettingen, Germany
- Tim van den Berg
- Department Medical Bioinformatics, University Medicine Goettingen, Goettingen, Germany
- Ivana Gavrilova
- Department of Gastroenterology, Gastrointestinal Oncology and Endocrinology, University Medicine Goettingen, Goettingen, Germany
- Ahmad Amanzada
- Department of Gastroenterology, Gastrointestinal Oncology and Endocrinology, University Medicine Goettingen, Goettingen, Germany
- Shiv K. Singh
- Department of Gastroenterology, Gastrointestinal Oncology and Endocrinology, University Medicine Goettingen, Goettingen, Germany
- Alaa Khalil
- Department of Gastroenterology, Gastrointestinal Oncology and Endocrinology, University Medicine Goettingen, Goettingen, Germany
- Rohia Alili
- Faculty of Medicine, Sorbonne University, INSERM UMRS NutriOmics, Paris, France
- Eugeni Belda
- Faculty of Medicine, Sorbonne University, INSERM UMRS NutriOmics, Paris, France
- Karine Clement
- Faculty of Medicine, Sorbonne University, INSERM UMRS NutriOmics, Paris, France
- Ahmed Abd El Wahed
- Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, Leipzig, Germany
- ElSagad Eltayeb Gady
- Faculty of Medicine, Al Neelain University/Ibn Sina Specialised Hospital, Khartoum, Sudan
- Martin Haubrock
- Department Medical Bioinformatics, University Medicine Goettingen, Goettingen, Germany
- Tim Beißbarth
- Department Medical Bioinformatics, University Medicine Goettingen, Goettingen, Germany
- Volker Ellenrieder
- Department of Gastroenterology, Gastrointestinal Oncology and Endocrinology, University Medicine Goettingen, Goettingen, Germany
- Albrecht Neesse
- Department of Gastroenterology, Gastrointestinal Oncology and Endocrinology, University Medicine Goettingen, Goettingen, Germany
- DOI
- https://doi.org/10.1128/mSystems.00750-21
- Journal volume & issue
-
Vol. 6,
no. 4
Abstract
Advanced microbiome analysis relies on sequencing of short DNA fragments from microorganisms like bacteria, fungi, and viruses. More recently, long fragment DNA sequencing of 3rd generation sequencing has gained increasing importance and can be rapidly conducted within a few hours due to its potential real-time sequencing.
