Prevalence of aminoglycoside resistance and ant(2”)-I gene in Pseudomonas aeruginosa isolated from burn wound specimens in Yazd

Abstract

Background: Pseudomonas aeruginosa is one of the most important causative agents among the hospital acquired infections, especially in ICU and burn units. Enzymatic inactivation of aminoglycosides by aminoglycoside-modifying enzymes is the main mechanism of resistance to these antibiotics in Pseudomonas aeruginosa. The aim of this study was to study the aminoglycoside resistance and ant (2”)-I in Pseudomonas aeruginosa isolated from burn specimens in Yazd, Iran. Materials and Methods: This cross-sectional study was carried out on Pseudomonas aeruginosa isolates (no=73) during July 2014 to April 2015. All burn wound samples were initially identified by the standard biochemical methods and their aminoglycoside resistance was studied using the disc diffusion method according to CLSI recommendations. PCR method was carried out for the detection of aminoglycoside resistance using ant (2”)-I gene specific primers. Results: Forty (54.8%) out of 73 cases were male (mean age 29±2.25 years). The resistance rates as determined by the disk diffusion method were: Kanamycin (89%), Gentamicin (67.1%), Tobramycin (58.9%) and Amikacin (60.3%). The PCR results showed that 63 (86.3%) of the isolates were harbored the ant (2”)-I gene. Conclusion: The results of this study show that resistance to aminoglycosides is high in pseudomonas aeruginosa isolated from burn wounds. The presence of gene ant (2”)-I was widely reported. In addition, there was a significant relationship between this gene and resistance to aminoglycosides.

Keywords

• Pseudomonas aeruginosa
• aminoglycoside resistance
• ant (2”)-I gene
• Burn wound