The Function of SD1 on Shoot Length and its Pyramiding Effect on Shoot Length and Plant Height in Rice (Oryza sativa L.)
Jingfang Dong,
Yamei Ma,
Haifei Hu,
Jian Wang,
Wu Yang,
Hua Fu,
Longting Zhang,
Jiansong Chen,
Lian Zhou,
Wenhui Li,
Shuai Nie,
Ziqiang Liu,
Junliang Zhao,
Bin Liu,
Tifeng Yang,
Shaohong Zhang
Affiliations
Jingfang Dong
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Yamei Ma
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Haifei Hu
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Jian Wang
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Wu Yang
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Hua Fu
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Longting Zhang
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Jiansong Chen
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Lian Zhou
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Wenhui Li
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Shuai Nie
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Ziqiang Liu
College of Agriculture, South China Agricultural University
Junliang Zhao
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Bin Liu
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Tifeng Yang
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Shaohong Zhang
Rice Research Institute, Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Engineering Laboratory, Key Laboratory of Genetics and Breeding of High -Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences
Abstract Strong seedling vigor is imperative to achieve stable seedling establishment and enhance the competitiveness against weeds in rice direct seeding. Shoot length (SL) is one of the important traits associated with seedling vigor in rice, but few genes for SL have been cloned so far. In the previous study, we identified two tightly linked and stably expressed QTLs for SL, qSL-1f and qSL-1d by genome-wide association study, and cloned the causal gene (LOC_Os01g68500) underlying qSL-1f. In the present study, we identify LOC_Os01g66100 (i.e. the semidwarf gene SD1), a well-known gene controlling plant height (PH) at the adult-plant stage, as the causal gene underlying qSL-1d through gene-based haplotype analysis and knockout transgenic verification. By measuring the phenotypes (SL and PH) of various haplotypes of the two genes and their knockout lines, we found SD1 and LOC_ Os01g68500 controlled both SL and PH, and worked in the same direction, which provided the directly genetic evidence for a positive correlation between SL and PH combined with the analysis of SL and PH in the diverse natural population. Moreover, the knockout transgenic experiments suggested that SD1 had a greater effect on PH compared with LOC_ Os01g68500, but no significant difference in the effect on SL. Further investigation of the pyramiding effects of SD1 and LOC_Os01g68500 based on their haplotype combinations suggested that SD1 may play a dominant role in controlling SL and PH when the two genes coexist. In this study, the effect of SD1 on SL at the seedling stage is validated. In total, two causal genes, SD1 and LOC_ Os01g68500, for SL are cloned in our studies, which controlled both SL and PH, and the suitable haplotypes of SD1 and LOC_ Os01g68500 are beneficial to achieve the desired SL and PH in different rice breeding objectives. These results provide a new clue to develop rice varieties for direct seeding and provide new genetic resources for molecular breeding of rice with suitable PH and strong seedling vigor.
