Evaluating Therapeutic Characteristics of Anticancer L -Glutaminase Enzyme on Ethylenimine Induced Mutagens in Rats.

Abstract

This study include, the partial purification of L-glutaminase enzyme from E.coli by ammonium sulphate precipitation (40%-80%) fractions, and the enzyme activity elevated to 75 IU/ml following dialysis. Pharmacological evaluation of L-glutaminase enzyme was used for the in vivo studies on sixty rats as an animal models. The hematological parameters for rats in the treated group (T2) showed significant changes at the level (P<0.05) when compared with normal control group (T1). The WBCs was found to be increased with the reduction in the RBCs and Hemoglobin (Hb.) concentration. However there were no significant changes of these parameters in the other treated groups (T3, T4, T5, and T6) in contrast to (T1). The serum total protein, globulin and, albumin were found to be decreased significantly for the (T2) in comparison with the (T1). However, their level had been relatively normalized in comparison to (T2) as a result of administrating doses of 0.5, and 1.0 ml L-glutaminase in combination with 0.5 mg ethylenimine /Kg. body weight for T5 and T6 respectively. Concerning the level of serum globulins, there were a significant elevation in the serum globulins value for T5 and T6 which were treated with doses of 0.5 and 1.0 ml L-glutaminase in combination with 0.5 mg ethylenimine/Kg. body weight respectively. While, a significant decrease in the values of serum albumin detected in all rats’ treated groups and the L-glutaminase efficacy was not achieved completely compared with the T1.The Liver enzymes ALT, AST and, ALP for T2 showed significant elevation at the level (p<0.05) when compared with T1. The treatment with L-glutaminase at doses of 0.5 and, 1 ml /kg body weight in combination with 0.5 mg of ethylenimine/kg body weight in T5 and, T6 groups respectively, seem to reverse the changes to word near normal level. There were a significant elevation (p<0.05) in the relative weight of Liver, kidney and spleen of rats in T2 in comparison with T1. However, there weight decreased significantly (p<0.05) in T5 group in comparison to (T2). Whereas there were no significant differences in the organs’ relative weight for the T6 group from that of T1. The finding of the present study improved the preventive role of the L-glutaminase against the mutagenic, and carcinogenic effect of ethylinimine, and it was dose dependent T1.

Keywords

• glutaminase
• anticancer
• ethylenimine
• coli