Microfluidics for the rapid detection of Staphylococcus aureus using antibody-coated microspheres

Abstract

Staphylococcus aureus is a common foodborne pathogenic microorganism which can cause food poisoning and it is pathogenic to both humans and animals. Therefore, rapid detection of S. aureus infection is of great significance. In this study, a microfluidic platform was introduced to detect S. aureus by fluorescence labeling method and a self-made microfluidic chip, which has immune spheres were used to study the effect of capturing S. aureus. Through this experiment, we found that the platform can be used for microbial culture, and S. aureus antibody coated on the diameter of 50 ~ 90 μm microspheres for detection. On the premise of optimizing the sample flow rate and detection time, the bacterial detection was quantitatively monitored. Results showed that our platform can detect S. aureus at injection rate of 5 μL·min−1 reacted for 4 min and the detection limit of bacteria is 1.5 × 101 CFU/μL. However, the detection time of traditional method is 24 hs to 72 hs, and the operation is complex and cumbersome. These findings indicated that the microfluidic chip in this study is portable, sensitive, and accurate, laying a good foundation for further research on the application of rapid bacterial detection platform.

Keywords

• microfluidic chip
• detection of staphylococcus aureus
• microspheres