EFFICACY OF COMBINED RECTAL CREAM “DILATIL” (DILTIAZEM/LIDOCAINE/METHYL-URACIL) IN PRE-CLINICAL STUDY ON A MODEL OF ACUTE COMPLICATED ANAL FISSURE IN COMPARISON WITH RECTAL CREAM NIFECAIN

Abstract

Aim: to assess efficacy of a new rectal cream “Dilatil” (diltiazem/lidocaine/methyluracil) (D) in pre-clinical study on a model of acute complicated anal fissure (ACAF) in compared to rectal cream “Nifecain” (nifedipine/lidocaine) (N). Materials and Methods: animal model of anal fissure, macroscopic assessment, enzyme-linked immunosorbent assay, statistical Student’s and Wilcoxon’s tests. Results: At the end of the study in the D group, the severity of the pathological process was statistically lower than in the Control pathology (CP) and N groups. Levels of IL-6, IL-10, and TNF-α in the homogenate of anodermal tissue (HAT) in the D group were significantly lower compared to CP. The level of IL-6 was significantly lower in the D group compared to N. Conclusions: better efficacy of D vs N was observed in a pre-clinical study that was also confirmed by the level of IL-10 in HAT tissue at the end of the experiment.

Keywords

• rectal cream
• anal fissure
• dilatil
• nifecain
• macroscopic assessment
• enzyme-linked immunosorbent assay
• il-10
• il-6
• hif-1α
• tnf-α
• calcium channel blockers
• diltiazem.