Nature Communications (Jan 2022)

Parallel functional assessment of m6A sites in human endodermal differentiation with base editor screens

  • Weisheng Cheng,
  • Fang Liu,
  • Zhijun Ren,
  • Wenfang Chen,
  • Yaxin Chen,
  • Tianwei Liu,
  • Yixin Ma,
  • Nan Cao,
  • Jinkai Wang

DOI
https://doi.org/10.1038/s41467-022-28106-0
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 13

Abstract

Read online

N6-methyladenosine (m6A) plays important role in lineage specifications of embryonic stem cells, but its role at specific sites has not been assessed. Here the authors develop an adenine editor-based strategy, and systematically identify functional m6A sites that control lineage decisions in human embryonic stem cells.