Measuring genetic diversity across populations.

Abstract

Diversity plays an important role in various domains, including conservation, whether it describes diversity within a population or diversity over a set of species. While various strategies for measuring among-species diversity have emerged (e.g. Phylogenetic Diversity (PD), Split System Diversity (SSD) and entropy-based methods), extensions to populations are rare. An understudied problem is how to assess the diversity of a collection of populations where each has its own internal diversity. Relying solely on measures that treat each population as a monomorphic lineage (like a species) can be misleading. To address this problem, we present four population-level diversity assessment approaches: Pooling, Averaging, Pairwise Differencing, and Fixing. These approaches can be used to extend any diversity measure that is primarily defined for a group of individuals to a collection of populations. We then apply the approaches to two measures of diversity that have been used in conservation-Heterozygosity (Het) and Split System Diversity (SSD)-across a dataset comprising SNP data for 50 anadromous Atlantic salmon populations. We investigate agreement and disagreement between these measures of diversity when used to identify optimal sets of populations for conservation, on both the observed data, and randomized and simulated datasets. The similarity and differences of the maximum-diversity sets as well as the pairwise correlations among our proposed measures emphasize the need to clearly define what aspects of biodiversity we aim to both measure and optimize, to ensure meaningful and effective conservation decisions.