Microbiologia Medica (May 2024)

Detection of Free-Living Amoeba in a tertiary care hospital

  • Nairita Choudhuri,
  • Bhaskar Narayan Chaudhuri,
  • Partha Guchhait,
  • Arup Kumar Dawn,
  • Satadal Das

DOI
https://doi.org/10.4081/mm.2024.12287
Journal volume & issue
Vol. 39, no. 1

Abstract

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This study aims to investigate the presence of Free-Living Amoebae (FLA), particularly Acanthamoeba spp., in diverse water systems and body fluids within a hospital. Water and body fluid samples were collected from various locations. Cultivation of FLA was performed using Non-Nutrient Agar (NNA) culture with Escherichia coli, and microscopic observations after staining. Microscopic observations revealed the presence of Acanthamoeba spp. in pre-dialysis tank water and dental flush water samples. The identification of Acanthamoeba in specific water sources underscores the importance of continuous monitoring and preventive strategies to mitigate potential health risks.

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