The World Journal of Men's Health (Jul 2022)
Sperm Morphology Assessment in the Era of Intracytoplasmic Sperm Injection: Reliable Results Require Focus on Standardization, Quality Control, and Training
- Ashok Agarwal,
- Rakesh Sharma,
- Sajal Gupta,
- Renata Finelli,
- Neel Parekh,
- Manesh Kumar Panner Selvam,
- Ralf Henkel,
- Damayanthi Durairajanayagam,
- Camila Pompeu,
- Sarah Madani,
- Andrea Belo,
- Neha Singh,
- Simryn Covarrubias,
- Sara Darbandi,
- Paraskevi Vogiatzi,
- Florence Boitrelle,
- Mara Simopoulou,
- Ramadan Saleh,
- Mohamed Arafa,
- Ahmad Majzoub,
- Hussein Kandil,
- Armand Zini,
- Edmund Ko,
- Juan G. Alvarez,
- Marlon Martinez,
- Jonathan Ramsay,
- Sunil Jindal,
- Gian Maria Busetto,
- Hassan Sallam,
- Israel Maldonado,
- Christina Anagnostopoulou,
- Marco G. Alves,
- Pallav Sengupta,
- Kambiz Gilany,
- Donald P. Evenson,
- Sheena E.M. Lewis,
- Jaime Gosalvez,
- Rafael F. Ambar,
- Rupin Shah
Affiliations
- Ashok Agarwal
- ORCiD
- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
- Rakesh Sharma
- ORCiD
- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
- Sajal Gupta
- ORCiD
- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
- Renata Finelli
- ORCiD
- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
- Neel Parekh
- ORCiD
- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
- Manesh Kumar Panner Selvam
- ORCiD
- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
- Ralf Henkel
- ORCiD
- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
- Damayanthi Durairajanayagam
- ORCiD
- Department of Physiology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Selangor, Malaysia, 7Instituto Ideia Fèrtil, Santo Andrè, Brazil
- Camila Pompeu
- ORCiD
- Instituto Ideia Fèrtil, Santo Andrè, Brazil
- Sarah Madani
- ORCiD
- Department of Biology and Physiology of Organisms, Faculty of Biological Sciences, University of Science and Technnology, Houari Boumedien, Algiers, Algeria
- Andrea Belo
- ORCiD
- Huntington Centro de Medicina Reproditiva S/A, Sao Paulo, São Paulo, Brazil
- Neha Singh
- ORCiD
- NOVA IVF, Gorakhpur, India
- Simryn Covarrubias
- ORCiD
- Department of Physiology, University of San Francisco, CA, USA
- Sara Darbandi
- ORCiD
- Fetal Health Research Center, Hope Generation Foundation, Tehran, Iran
- Paraskevi Vogiatzi
- ORCiD
- Andromed Health & Reproduction, Fertility Diagnostics Laboratory, Maroussi, Athens, Greece
- Florence Boitrelle
- ORCiD
- Reproductive Biology, Fertility Preservation, Andrology, CECOS, Poissy Hospital, Poissy
- Mara Simopoulou
- ORCiD
- Department of Experimental Physiology, School of Health Sciences, Faculty of Medicine, National and Kapodistrian University of Athens, Athens, Greece
- Ramadan Saleh
- ORCiD
- Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Sohag University, Sohag, Egypt
- Mohamed Arafa
- ORCiD
- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
- Ahmad Majzoub
- ORCiD
- American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
- Hussein Kandil
- ORCiD
- Fakih IVF Fertility Center, Abu Dhabi, UAE, 21Department of Surgery, McGill University, Montreal, QC, Canada
- Armand Zini
- ORCiD
- Department of Surgery, McGill University, Montreal, QC, Canada
- Edmund Ko
- ORCiD
- Department of Urology, Loma Linda University Health, Loma Linda, CA, USA
- Juan G. Alvarez
- ORCiD
- Centro ANDROGEN, La Coruña, Spain
- Marlon Martinez
- ORCiD
- Section of Urology, University of Santo Tomas Hospital, Manila, Philippines
- Jonathan Ramsay
- ORCiD
- Department of Andrology, Hammersmith Hospital, London, UK,
- Sunil Jindal
- ORCiD
- Department of Andrology and Reproductive Medicine, Jindal Hospital, Meerut, India
- Gian Maria Busetto
- ORCiD
- Department of Urology and Renal Transplantation, University of Foggia Policlinico Riuniti of Foggia, Foggia, Italy
- Hassan Sallam
- ORCiD
- Department of Obstetrics and Gynaecology, Alexandria University Faculty of Medicine, Alexandria, Egypt
- Israel Maldonado
- ORCiD
- Citmer Reproductive Medicine, IVF LAB, Mexico City, Mexico
- Christina Anagnostopoulou
- ORCiD
- IVF Clinic “Akeso-Embryo ART”, Athens, Greece
- Marco G. Alves
- ORCiD
- Department of Anatomy, Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal
- Pallav Sengupta
- ORCiD
- Department of Physiology, Faculty of Medicine, MAHSA University, Selangor, Malaysia
- Kambiz Gilany
- ORCiD
- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
- Donald P. Evenson
- ORCiD
- SCSA Diagnostics, Brookings, SD, USA
- Sheena E.M. Lewis
- ORCiD
- Queens University Belfast, Weavers Court, Belfast, Northern Ireland, UK
- Jaime Gosalvez
- ORCiD
- Genetic Unit, Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain
- Rafael F. Ambar
- ORCiD
- Department of Urology, Centro Universitario em Saude do ABC/Andrology Group at Ideia Fertil Institute of Human Reproduction, Santo André, Brazil
- Rupin Shah
- ORCiD
- Department of Urology, Lilavati Hospital and Research Centre, Mumbai, India
- DOI
- https://doi.org/10.5534/wjmh.210054
- Journal volume & issue
-
Vol. 40,
no. 3
pp. 347 – 360
Abstract
Semen analysis is the first, and frequently, the only step in the evaluation of male fertility. Although the laboratory procedures are conducted according to the World Health Organization (WHO) guidelines, semen analysis and especially sperm mor-phology assessment is very difficult to standardize and obtain reproducible results. This is mainly due to the highly subjective nature of their evaluation. ICSI is the choice of treatment when sperm morphology is severely abnormal (teratozoospermic). Hence, the standardization of laboratory protocols for sperm morphology evaluation represents a fundamental step to ensure reliable, accurate and consistent laboratory results that avoid misdiagnoses and inadequate treatment of the infertile patient. This article aims to promote standardized laboratory procedures for an accurate evaluation of sperm morphology, including the establishment of quality control and quality assurance policies. Additionally, the clinical importance of sperm morphol-ogy results in assisted reproductive outcomes is discussed, along with the clinical management of teratozoospermic patients.
Keywords
