Effects and Regulatory Mechanisms of SNAI2 on Malignant Hallmarks of Non-Invasive Breast Tumor Cells

Abstract

Objective To explore the effects and related mechanisms of SNAI2 on malignant hallmarks of non-invasive breast tumor MCF-7 and T-47D cells. Methods (1) The lentiviral vector was used to screen MCF-7 and T-47D cells with SNAI2 overexpression; (2) The proliferation, migration and anti-apoptosis abilities of tumor cells were detected by MTT assay, wound healing and Hoechst 33342 assay, respectively; (3) The gene expression was tested by real-time PCR. Results (1) The GV367-SNAI2 was successfully transfected into MCF-7 and T-47D cells; (2)The SNAI2 could enhance malignant hallmarks of non-invasive breast tumor cells, including the proliferation, migration and anti-apoptosis abilities(P < 0.01); (3) The over-expression of SNAI2 could up-regulate the expression of Cyclin D1, MMP9, VIM and down-regulate the expression of E-CDH in MCF-7 and T-47D cells(P < 0.01). Conclusion SNAI2 could promote higher metastasis potential of non-invasive breast tumor cells through EMT, which will provide the experimental foundation for further exploring new targeted therapeutic method.

Keywords

• signal transcription factor snai2
• lentivirus vector
• metastatic