Anti-metastatic effect of curcumin analog pentagamaboronon-0-fructose (PGB-0-F) against 4T1 breast cancer cells

Abstract

Development of chemotherapeutic agent and boron carrying pharmaceutical based on triple negative breast cancer is important due to its metastatic progression. Metastases are often more dangerous than the primary tumor and they are responsible for 90% of all cancer deaths. The purpose of this study is to explore the anti-metastatic activities of the PGB-0 complex with fructose (PGB-0-F) against 4T1 breast cancer cells. Scratch wound healing assay was performed to determine migration inhibition ability of PGB-0-F, while MMP-9 expression were analysed by gelatin zymography. The testing of anti-migration activity showed that PGB-0-F inhibited in 4T1 cells, whereas in gelatin zymography assay showed the suppression of MMP-9 expression. PGB-0-F inhibited closure on 4T1 metastatic breast cancer cells line compared to control. PGB-0-F decreased MMP-9 expression level compared to control. Based these results, PGB-0-F has a potency to be developed as chemotherapeutic agent especially as anti-metastatic agent

Keywords

• curcumin analogue, PGB-0-F, 4T1 Cells, migration, MMP-9