The LC-QTOF-MS/MS analysis of acid degradation products of Rifaximin, an antibiotic
Saima Baig Yaseen,
Arfa Akram,
Syed Ghulam Musharraf,
Mehwish Wajidi,
Nargis Tabassum,
Nemat Nazir,
Syed Muhammad Zaki Shah
Affiliations
Saima Baig Yaseen
Federal Urdu University of Arts Sciences and Technology, Karachi, Sindh Pakistan; Corresponding author.
Arfa Akram
Federal Urdu University of Arts Sciences and Technology, Karachi, Sindh Pakistan
Syed Ghulam Musharraf
H.E.J Research Institute of Chemistry, International Center for chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan; Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan
Mehwish Wajidi
Federal Urdu University of Arts Sciences and Technology, Karachi, Sindh Pakistan
Nargis Tabassum
Federal Urdu University of Arts Sciences and Technology, Karachi, Sindh Pakistan
Nemat Nazir
Federal Urdu University of Arts Sciences and Technology, Karachi, Sindh Pakistan
Syed Muhammad Zaki Shah
H.E.J Research Institute of Chemistry, International Center for chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan
The present research aims to propose a simple and accurate technique for the analysis of Rifaximin in the presence of its stress degradation products and analysis of degradation products by LC-MS/MS analysis. Rifaximin was submitted to forced degradation under the acid hydrolysis condition as prescribed by the ICH. The extract was prepared by firstly treated with HCl and heated about 4 to 8 h. The filtrate was collected and separated using dichloromethane followed by evaporation in rotary evaporator to obtain a solid crude extract which was then stored under refrigeration at -80 °C. Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS/MS) was utilized to identify products in the drug sample. The data processing results revealed the presence of 9 products in the degraded sample of Rifaximin. This data article contains the m/z [M + H +] values, molecular formula, retention times and the comprehensive list of m/z values detected during the LC- QTOF- MS/MS analysis.
