Elaboration of Methods for Detection of Xanthomonas axonopodis pv. phaseoli on Bean Seeds

Abstract

Xanthomonas axonopodis pv. phaseoli detection on artificially inoculated bean seeds was investigated. The method of the International Seed Federation – ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X. a. pv. phaseoli.

Keywords

• Xanthomonas axonopodis pv. phaseoli
• Bean
• Seed
• Artificial inoculation
• ELISA
• PCR