Leclercia adecarboxylata From Human Gut Flora Carries mcr-4.3 and blaIMP-4-Bearing Plasmids

Qiaoling Sun; Hanyu Wang; Lingbin Shu; Ning Dong; Ning Dong; Fan Yang; Hongwei Zhou; Sheng Chen; Sheng Chen; Rong Zhang

doi:10.3389/fmicb.2019.02805

Frontiers in Microbiology (Dec 2019)

Leclercia adecarboxylata From Human Gut Flora Carries mcr-4.3 and blaIMP-4-Bearing Plasmids

Qiaoling Sun,
Hanyu Wang,
Lingbin Shu,
Ning Dong,
Ning Dong,
Fan Yang,
Hongwei Zhou,
Sheng Chen,
Sheng Chen,
Rong Zhang

Affiliations

Qiaoling Sun: Department of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China
Hanyu Wang: University of Conneticut, Mansfield, CT, United States
Lingbin Shu: Department of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China
Ning Dong: Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, China
Ning Dong: State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, China
Fan Yang: Department of Biophysics and Kidney Disease Center, The First Affiliated Hospital, Institute of Neuroscience, National Health Commission and Chinese Academy of Medical Sciences Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
Hongwei Zhou: Department of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China
Sheng Chen: Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, China
Sheng Chen: State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, China
Rong Zhang: Department of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China

DOI: https://doi.org/10.3389/fmicb.2019.02805
Journal volume & issue: Vol. 10

Abstract

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A clinical Leclercia adecarboxylata strain harboring the mcr-4.3 and blaIMP-4 genes was isolated from active rectal screening of carbapenem-resistant Enterobacteriaceae (CRE) in a patient. The isolate was found to harbor seven plasmids, including a 94,635 bp blaIMP-4-bearing IncN plasmid and a 9,782 bp mcr-4.3-bearing ColE10-type plasmid. The isolate was susceptible to colistin despite carrying the mcr-4.3 gene, suggesting that this MCR-4 variant may not be functional. Carriage of antibiotic resistance genes in human gut L. adecarboxylata strain suggests that close surveillance of resistance strains in the human gut flora should be included as a routine clinical practice to prevent occurrence of infections, especially among immunocompromised patients.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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