Association of c.*49T>C and c.-932 G>A Polymorphisms of CYBA Gene with Coronary Artery Disease: a Case-Control Study in Kashan Population

Abstract

Background: The CYBA (Cytochrome B-245 Alpha Chain; p22phox) gene encodes an essential subunit of NADH/NADPH-oxidase This enzyme expressed in smooth muscle cells of arterieswhich produces the active oxygen species. On the other hand oxidative stress has a significant role in the pathogenesis of coronary artery disease (CAD). The aim of this study was to investigate the association of rs7195830 (c.*49T>C) and rs9932581 (c.-930G>A) polymorphisms in CYBA gene with coronary artery disease in an Iranian population. Materials and Methods: In this case-control study, citrated blood samples were obtained from 180 subjects including 85 patient with CAD and 95 healthy people. The fragments containing rs7195830 and rs9932581 of CYBA gene from extracted genome were amplified by polymerase chain reaction. Then the genotype of samples was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genetic association analysis was assessed by logistic regression test. Results: Data analysis of c.-930G>A polymorphism revealed a significant association between AA genotype and risk of CAD (OR: 2.71, 95%CI: 1.04-7.06, p= 0.041). Also, allelic analysis revealed that there was a significant association between A allele and CAD risk (OR: 1.65, 95%CI: 1.05-2.57, p=0.029), while there was no significant association between c.*49T>C polymorphism and risk of CAD. Conclusion: Findings showed that, the c.-930G>A polymorphism may have some role in the susceptibility to CAD. which it can consider as a potential biomarker in further researches.

Keywords

• Coronary artery disease
• CYBA gene
• Genetic polymorphism
• Kashan