Modulatory effects of selected compounds on oxidative stress in hydrogen peroxide-induced Drosophila melanogaster

Abstract

The balance between oxidant production and their conversion to harmless products is vital to the survival of living organisms. Hence the comparative free radical scavenging activity of quercetin, caffeic acid, ascorbic acid and gallic acid was studied using in vitro, in silico and in vivo model of Drosophila melanogaster. The FRAP and DPPH scavenging activities was performed using standard protocol, the molecular docking of the compounds against the active site of glutathione-s-transferase-2 was carried out using autodoc vina (v11-1). Hydrogen peroxide-induced D. melanogaster was use for evaluation of antioxidant parameters using standard procedures. The results revealed that, ascorbic acid has the highest percentage DPPH inhibition across the concentration with no significant difference in the ability of the compounds to reduce Fe3+ to Fe2+. The antioxidant compounds ameliorate weight loss and decrease percentage negative geotaxis observed in H2O2-induced flies without treatment. Data from this study showed that, the compounds mitigated against H2O2-induced accumulation of nitric acid, hydrogen peroxide and malondialdehyde. Moreover, the compounds improved total thiol and non-protein thiol concentration compared with H2O2 induced flies. The in silico study showed that, quercetin has the highest binding affinity more than the standard drug (methyldopa). All the compounds showed better reactivity potential compared with methyldopa. In conclusion, all the compounds offer protection against H2O2-induced oxidative stress in D. melanogaster.

Keywords

• Antioxidants
• In vitro
• In vivo
• In silico
• Hydrogen peroxide