Quantitative proteomic dataset of whole protein in three melanoma samples of 92.1, 92.1-A and 92.1-B
Xifeng Fei,
Xiangtong Xie,
Xiaoyan Ji,
Haiyan Tian,
Fei Sun,
Dongyi Jiang,
Zhimin Wang,
Qiang Huang
Affiliations
Xifeng Fei
Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, China
Xiangtong Xie
Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, China
Xiaoyan Ji
Department of Ophthalmology, Second Affiliated Hospital of Suzhou Medical College of Soochow University, China; Corresponding author.
Haiyan Tian
Department of GCP, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, China
Fei Sun
Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, China
Dongyi Jiang
Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, China
Zhimin Wang
Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, China; Department of Neurosurgery, Second Affiliated Hospital of Suzhou Medical College of Soochow University, China; Corresponding author at: Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, 118 Wanshen Street, Suzhou, China
Qiang Huang
Department of GCP, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, China
Distant metastasis is common in ocular uveal melanoma (uveal melanoma, UM) [1], with possible identification of relevant protein markers in peripheral blood [2,3]. Proteomics analyses serve as a basis for the screening of new target proteins. However, it is difficult to determine whether the relevant proteins in peripheral blood are the same kinesins as those in primary lesions and metastases. Specially in this study, human UM cells (92.1) [4] were inoculated into the back of the eyeball and the brain of inbred line nude mice transplanted with enhanced green fluorescent protein (EGFP) [5], respectively, to simulate the growth of UM in situ and in brain metastases. A database was established as follows: Firstly, the xenograft was taken for monoclonal re-culture and amplification. Then, the cells after amplification (92.1-A in the back of the eyeball and 92.1-B in the brain) and their parent cells (92.1) were subjected to Tandem Mass Tag (TMT)-labeling proteomic analysis and liquid chromatography-mass spectrometry (LC-MS). Covering differential proteomes of three cell lines in a pairwise model, the data could be used to further screen the kinesins that play a vital role in regulating the growth of UM.
