Engineering an incubation environment that mimics in situ conditions for in vitro coastal microbiome studies
Brad T Hunter,
Jaeden D Flury,
Sebastian S Cocioba,
Matthew L Cope-Arguello,
Jared M Helms,
Kevin H García,
Gerardo Dominguez,
Darcy AA Taniguchi,
Elinne Becket
Affiliations
Brad T Hunter
1College of Science, Technology, Engineering, & Mathematics, California State University San Marcos, 333 S. Twin Oaks Valley Rd, San Marcos, CA 92096, USA
Jaeden D Flury
1College of Science, Technology, Engineering, & Mathematics, California State University San Marcos, 333 S. Twin Oaks Valley Rd, San Marcos, CA 92096, USA
Sebastian S Cocioba
2Binomica Labs, Long Island City, NY, 4301 22nd St fl 3rd studio 342, Long Island City, NY 11101, USA
Matthew L Cope-Arguello
1College of Science, Technology, Engineering, & Mathematics, California State University San Marcos, 333 S. Twin Oaks Valley Rd, San Marcos, CA 92096, USA
Jared M Helms
1College of Science, Technology, Engineering, & Mathematics, California State University San Marcos, 333 S. Twin Oaks Valley Rd, San Marcos, CA 92096, USA
Kevin H García
1College of Science, Technology, Engineering, & Mathematics, California State University San Marcos, 333 S. Twin Oaks Valley Rd, San Marcos, CA 92096, USA
Gerardo Dominguez
1College of Science, Technology, Engineering, & Mathematics, California State University San Marcos, 333 S. Twin Oaks Valley Rd, San Marcos, CA 92096, USA
Darcy AA Taniguchi
1College of Science, Technology, Engineering, & Mathematics, California State University San Marcos, 333 S. Twin Oaks Valley Rd, San Marcos, CA 92096, USA
Elinne Becket
1College of Science, Technology, Engineering, & Mathematics, California State University San Marcos, 333 S. Twin Oaks Valley Rd, San Marcos, CA 92096, USA
Coastal environments are dynamic and can vary widely on short- or long-term scales depending on location and weather. Incubation equipment that reflects these changes through programmable gradient light and temperature cycles would permit more precise in vitro coastal microbiome studies. Here we present an open-source incubation environment that mimics in situ conditions for in vitro coastal microbiome studies using a modified shaking water bath that has fully customizable temperature and light gradients that can also mimic real-time field conditions. We compared coastal microbial community profiles incubated in situ and in our build mimicking field conditions over 48 h. Analyses of congruence indicated significant overlap (p > 0.2) between microbial communities incubated in situ and in vitro at each time point.
