Improvement of Phenylpropanoid Production with Elicitor Treatments in <i>Pimpinella brachycarpa</i> Nakai

Abstract

Pimpinella brachycarpa Nakai, known as cham-na-mul in Korea, is a popular edible herb and vegetable. Phenolic compounds are recognized as a vital group of plant secondary metabolites that provide innumerable, valuable therapeutic properties. Elicitors are biofactors or chemicals from diverse sources that can trigger morphological and physiological responses in the target organism. This study examined the effect of methyl jasmonate (MeJA), salicylic acid (SA), and chitosan treatment on the accretion of phenolic compounds in P. brachycarpa Nakai. This plant was harvested under different concentration of elicitor treatment for time course. Eight phenolic compounds including were detected in response to elicitor using HPLC. While the untreated controls showed the lowest phenolic content, treatment with 0.3% chitosan, 0.1 mM SA, and 0.1 mM MeJA resulted in 1.43-, 1.39-, and 1.35-fold increase in the phenolic content, respectively. The highest content of most of the individual phenolic compounds followed a similar trend according to treatment type, with chitosan treatment showing the highest content, followed by SA and then MeJA treatments. Thus, we demonstrate that the treatment with optimal concentrations of these elicitors for an optimal period of time increases the production of phenolic compounds in P. brachycarpa Nakai.

Keywords

• <i>Pimpinella brachycarpa</i> nakai
• elicitors
• phenolic compounds
• methyl jasmonate
• salicylic acid
• chitosan