Nature Communications (Apr 2023)
Single cell transcriptomic analysis of HPV16-infected epithelium identifies a keratinocyte subpopulation implicated in cancer
- Mary C. Bedard,
- Tafadzwa Chihanga,
- Adrean Carlile,
- Robert Jackson,
- Marion G. Brusadelli,
- Denis Lee,
- Andrew VonHandorf,
- Mark Rochman,
- Phillip J. Dexheimer,
- Jeffrey Chalmers,
- Gerard Nuovo,
- Maria Lehn,
- David E. J. Williams,
- Aditi Kulkarni,
- Molly Carey,
- Amanda Jackson,
- Caroline Billingsley,
- Alice Tang,
- Chad Zender,
- Yash Patil,
- Trisha M. Wise-Draper,
- Thomas J. Herzog,
- Robert L. Ferris,
- Ady Kendler,
- Bruce J. Aronow,
- Matthew Kofron,
- Marc E. Rothenberg,
- Matthew T. Weirauch,
- Koenraad Van Doorslaer,
- Kathryn A. Wikenheiser-Brokamp,
- Paul F. Lambert,
- Mike Adam,
- S. Steven Potter,
- Susanne I. Wells
Affiliations
- Mary C. Bedard
- Division of Oncology, Cincinnati Children’s Hospital Medical Center
- Tafadzwa Chihanga
- Division of Oncology, Cincinnati Children’s Hospital Medical Center
- Adrean Carlile
- Division of Oncology, Cincinnati Children’s Hospital Medical Center
- Robert Jackson
- School of Animal and Comparative Biomedical Sciences, University of Arizona
- Marion G. Brusadelli
- Division of Clinical Operations, Medpace
- Denis Lee
- McArdle Laboratory for Cancer Research, School of Medicine and Public Health, University of Wisconsin
- Andrew VonHandorf
- Center for Autoimmune Genomics and Etiology (CAGE), Cincinnati Children’s Hospital Medical Center
- Mark Rochman
- Division of Allergy and Immunology, Cincinnati Children’s Hospital Medical Center
- Phillip J. Dexheimer
- Division of Biomedical Informatics, Cincinnati Children’s Hospital Medical Center
- Jeffrey Chalmers
- William G. Lowrie Department of Chemical and Biomolecular Engineering, Ohio State University
- Gerard Nuovo
- Department of Pathology, Ohio State University Medical Center
- Maria Lehn
- Division of Hematology/Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine
- David E. J. Williams
- Cancer Biology Graduate Interdisciplinary Program, University of Arizona
- Aditi Kulkarni
- UPMC Hillman Cancer Center, University of Pittsburgh
- Molly Carey
- Department of Obstetrics and Gynecology, University of Cincinnati Medical Center
- Amanda Jackson
- Department of Obstetrics and Gynecology, University of Cincinnati Medical Center
- Caroline Billingsley
- Department of Obstetrics and Gynecology, University of Cincinnati Medical Center
- Alice Tang
- Department of Otolaryngology, University of Cincinnati
- Chad Zender
- Department of Otolaryngology, University of Cincinnati
- Yash Patil
- Department of Otolaryngology, University of Cincinnati
- Trisha M. Wise-Draper
- Division of Hematology/Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine
- Thomas J. Herzog
- Department of Obstetrics and Gynecology, University of Cincinnati Medical Center
- Robert L. Ferris
- UPMC Hillman Cancer Center, University of Pittsburgh
- Ady Kendler
- Department of Pathology & Laboratory Medicine, University of Cincinnati College of Medicine
- Bruce J. Aronow
- Division of Biomedical Informatics, Cincinnati Children’s Hospital Medical Center
- Matthew Kofron
- Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center
- Marc E. Rothenberg
- Division of Allergy and Immunology, Cincinnati Children’s Hospital Medical Center
- Matthew T. Weirauch
- Center for Autoimmune Genomics and Etiology (CAGE), Cincinnati Children’s Hospital Medical Center
- Koenraad Van Doorslaer
- School of Animal and Comparative Biomedical Sciences, University of Arizona
- Kathryn A. Wikenheiser-Brokamp
- Department of Pathology & Laboratory Medicine, University of Cincinnati College of Medicine
- Paul F. Lambert
- McArdle Laboratory for Cancer Research, School of Medicine and Public Health, University of Wisconsin
- Mike Adam
- Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center
- S. Steven Potter
- Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center
- Susanne I. Wells
- Division of Oncology, Cincinnati Children’s Hospital Medical Center
- DOI
- https://doi.org/10.1038/s41467-023-37377-0
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 20
Abstract
The role of keratinocyte subpopulations in the different phases of the viral cycle during HPV16 infection remains to be characterised. Here, single cell RNA sequencing of HPV16 infected and uninfected organoids identifies 12 distinct keratinocyte populations including an HPV-reprogrammed keratinocyte subpopulation that is linked to cancer.
