RNA-seq reveals novel mechanistic targets of Livin in bladder cancer

Abstract

Abstract Background Bladder cancer is a very common malignancy with a high recurrence rate. The survival of patients with muscle-invasive bladder cancer is poor, and new therapies are needed. Livin has been reported to be upregulated in bladder cancer and influence the proliferation of cancer cells. Materials and methods The Livin gene in human bladder cancer cell line T24 was knocked out, and the differentially expressed genes were identified by RNA-seq and qPCR. Results Livin knockdown affects gene expression and has strong negative effects on some cancer-promoting pathways. Furthermore, combined with bladder cancer clinical sample data downloaded from TCGA and GEO, 2 co-up-regulated genes and 58 co-down-regulated genes were identified and validated, which were associated with cancer proliferation and invasion. Conclusion All these results suggest that Livin plays an important role in bladder cancer and could be a potential anticancer target in clinical therapy.

Keywords

• Bladder cancer
• Livin
• T24 cell
• RNA-seq