Cancer Management and Research (Jan 2021)
GSG2 Promotes Development and Predicts Poor Prognosis of Ovarian Cancer
Abstract
Yan Huang,1,2,* Yixuan Liu,2,3,* Keyu Zhu,2,3,* Xiaolu Ma,2,3 Renquan Lu,2,3 Meiqin Zhang1,2 1Department of Gynecologic Oncology, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, People’s Republic of China; 2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of China; 3Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Renquan LuDepartment of Clinical Laboratory, Fudan University Shanghai Cancer Center, 270 Dong’An Road, Shanghai 200032, People’s Republic of ChinaTel +86 18017312789Email [email protected] ZhangDepartment of Gynecologic Oncology, Fudan University Shanghai Cancer Center, 270 Dong’An Road, Shanghai 200032, People’s Republic of ChinaTel +86 18017312218Fax +86 21-64175590Email [email protected]: Ovarian cancer is one of the most common malignant tumors in gynecology, whose treatment was seriously limited by the unclear understanding of molecular mechanism in disease development. GSG2, also known as Haspin, is a novel molecule found to be involved in human cancers.Materials and Methods: In this study, immunohistochemical analysis was used to detect GSG2 expression in ovarian cancer tissues and corresponding normal tissues. Statistical analysis was performed to construct relationship between GSG2 and tumor characteristics as well as prognosis. Ovarian cell model with GSG2 knockdown was constructed through lentivirus-mediated transfection of shRNA, which was used in MTT assay, colony formation assay and flow cytometry for investigating the role of GSG2 in ovarian cancer. A human apoptosis antibody array was used to identify potential downstream apoptosis-related proteins of GSG2.Results: The results demonstrated the upregulation of GSG2 in ovarian cancer, whose expression was positively related to tumor grade and AJCC stage, and negatively correlated with patients’ prognosis. Moreover, knockdown of GSG2 inhibited ovarian cancer development through suppressing cell growth and inducing cell apoptosis. Further exploration revealed that a variety of apoptosis-related and PI3K signaling pathway-related proteins may be implicated in the GSG2 induced regulation of ovarian cancer.Conclusion: In summary, it was illustrated that GSG2 was involved in the development of ovarian cancer, which has the potential to become therapeutic target and prognostic indicator in ovarian cancer treatment.Keywords: ovarian cancer, GSG2, prognosis, cell proliferation, cell apoptosis
