Changes of biochemical structure of gastric mucosa with intraepithelial neoplasia by fiber Raman system

Abstract

Objective To observe the biochemical structural changes of gastric mucosa with intraepithelial neoplasia by fiber Raman system. Methods Postoperative specimens of endoscopic submucosal dissection (ESD)-treated patients were measured to get Raman spectra by fiber Raman spectroscopy system. There were 6 ESD specimens with low-grade intraepithelial neoplasia (LGIN) and 5 with high-grade intraepithelial neoplasia (HGIN). A total of 51 sets of standard Raman spectral data were obtained by the fiber Raman system, including 16 sets of low-grade intraepithelial neoplasia tissue data, 15 sets of high-grade intraepithelial neoplasia tissue data, and 20 sets of non-lesional tissue data. After data conversion and a series of data preprocessing steps, the average Raman spectrum was obtained. After analysis of 3 sets of image patterns and Raman peak assignment, further analysis in the changes of biochemical structure of gastric mucosa with intraepithelial neoplasia were carried out. Results Compared with non-lesional tissues, peaks at 852 cm-1, 891 cm-1, 992 cm-1, 1 103 cm-1, 1 171 cm-1, 1 234 cm-1, 1 417 cm-1, 1 560 cm-1 and 1 586 cm-1 shifted to the same direction, and the magnitudes of shift was positively correlated with the severity of tumor transformation. Characteristic Raman peaks were observed at 805 cm-1, 819 cm-1, 1 306 cm-1 and 1 327 cm-1 in low-grade intraepithelial neoplasia tissues, and 875 cm-1, 1 304 cm-1 and 1 324 cm-1 in high-grade intraepithelial neoplasia tissues, which suggesting that the biochemical structure and molecular expression of gastric mucosa changed with the occurrence and advance of intraepithelial neoplasia. Conclusion Raman spectroscopy indicates that the gene structure changes during tumorigenesis, and some nucleic acid bases and proteins are highly expressed. The above changes can be reflected intraepithelial neoplasia of gastric mucosa.

Keywords

• raman spectroscopy
• intraepithelial neoplasia
• endoscopic submucosal dissection specimen
• differentiation