Advances in Cancer Biology - Metastasis (Dec 2021)
Evaluation of oral cancer associated glycosyltransferase expression levels in peripheral blood mononucleated cells
Abstract
Aberrant glycosylation, particularly sialylation is considered a significant characteristic of malignancy. Aberrant sialylation has been connected with several neoplastic disorders. The role of abnormal sialylation in oral cancer blood, on the other hand, is still in its early stages. The present study focussed to examine relative quantification of mRNA expression levels of Dolichyl-Phosphate N-Acetylglucosaminephosphotransferase 1 (DPAGT1) encoding enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycosylation, Beta-1,4-Mannosyl-Glycoprotein 4-Beta-N-Acetylglucosaminyltransferase (MGAT3) and Alpha-1,6-Mannosylglycoprotein 6-Beta-N-Acetylglucosaminyltransferase (MGAT5) mediated N-glycosylation, selected α2, 3, α2, 6 sialyltransferases families and sialidase in thirty human oral cancer blood and twelve control samples by Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) method. The results indicated significant upregulation of MGAT3, ST3 Beta-Galactoside Alpha-2, 3-Sialyltransferase 1 (ST3GAL1), ST6 N-Acetylgalactosaminide Alpha-2, 6-Sialyltransferase 1(ST6GALNAC1), and Neuraminidase 3 (NEU3) compared to healthy controls. In terms of habits, tobacco chewing and smoking, high expression levels of ST3GAL1 and ST6 Beta-Galactoside Alpha-2, 6-Sialyltransferase 1 (ST6GAL1) were observed. Although, MGAT3 and NEU3 elevated expression were statistically significant in patients who had no habits. The expressions of DPAGT1, MGAT3, MGAT5, ST3GAL1, ST3 Beta-Galactoside Alpha-2, 3-Sialyltransferase 6 (ST3GAL6), ST6GALNAC1, and NEU3 were also linked to the disease severity and progression. Hence, this study demonstrated the significant role of elevated mRNA levels of ST3GAL1, ST6GALNAC1, and NEU3 in peripheral blood mononucleated cells of oral cancer disease progression.
