Response to the application of Pectimorf® and Viyoya® to coffee tree seedlings regenerated from germplasm conserved in vitro during the acclimatization phase

Abstract

The employment of biotechnological methods in conservation and multiplication of promissory genotypes of coffee is significant, but one of the limitations is the management of vitroplants during the adaptation phase. The aim of this research was to analyse the response of an F1 hybrid seedlings of coffee to the acclimatization phase with the application of new biofertilizers, respectively Pectimorf® and Viyoya®. As plant material seedlings of hybrid F1 H-434 obtained by somatic embryogenesis were used. The effect of Pectimorf® was determined in interaction with Rhizophagus intraradices and Funneliformis mosseae. In the second experiment, the growth and development of coffee seedlings was evaluated, with a biofertilizer based on efficient microorganisms (Viyoya®) combined with arbuscular mycorrhizal fungi. The study was carried out under greenhouse temperature of 25 °C and relative humidity of 75%. The results showed positive responses to the acclimatization of the seedlings with the bioproducts, in nutrition and growth. Concentrations of 10-20 mg L-1 of Pectimorf® guaranteed significant average increases for height, stem diameter and pairs of leaves, in combination with the R. intraradices strain (T3, T5). In addition, it had been seen that a favourable percentage of infestation was obtained in the soil used. The treatments with 1 and 3 mL of Viyoya® in combination with the AMF strains showed an increase in the indicators of plant height, leaf pairs and stem diameter, as well as the leaf area, the values being higher when applying 3 mL of Viyoya® (T3, T4, T5, T6). These results demonstrate the usefulness of plant growth promoting products in the acclimatization phase of an F1 hybrid seedlings of coffee.

Keywords

• acclimatization
• Coffea arabica L.
• HMA
• in vitro culture
• Pectimorf