Proceedings
(Aug 2017)
Leishmania spp. Detection Using a Surface Plasmon Resonance Biosensor
Edlaine Ferreira,
Jefferson Lima,
Renata Pereira Alves-Balvedi,
Paulo Rogério Bonan,
Eliton Medeiros,
Luiz Goulart,
Antonio Lima,
Helmut Neff,
Leiva Oliveira,
Lucio Castellano,
Artur Araújo,
Cleumar Moreira
Affiliations
Edlaine Ferreira
Human Immunology Research and Education Group, Universidade Federal da Paraíba, 58051-900 PB, Brazil
Jefferson Lima
Human Immunology Research and Education Group, Universidade Federal da Paraíba, 58051-900 PB, Brazil
Renata Pereira Alves-Balvedi
Institute of Genetics and Biochemistry. Universidade Federal de Uberlândia, Uberlândia, 38408-100 MG, Brazil
Paulo Rogério Bonan
Human Immunology Research and Education Group, Universidade Federal da Paraíba, 58051-900 PB, Brazil
Eliton Medeiros
Materials Science Engineering Department, Universidade Federal da Paraíba, Joao Pessoa, 58051-900 PB, Brazil
Luiz Goulart
Institute of Genetics and Biochemistry. Universidade Federal de Uberlândia, Uberlândia, 38408-100 MG, Brazil
Antonio Lima
Electrical Engineering Department. Universidade Federal de Campina Grande, 58051-900 Paraiba, Brazil
Helmut Neff
Electrical Engineering Department. Universidade Federal de Campina Grande, 58051-900 Paraiba, Brazil
Leiva Oliveira
Universidade Federal Rural do Semi-Árido, Angicos, 59515-000 RN, Brazil
Lucio Castellano
Human Immunology Research and Education Group, Universidade Federal da Paraíba, 58051-900 PB, Brazil
Artur Araújo
Université de Lorraine, 54000 Nancy, France
Cleumar Moreira
Electrical Engineering Department. Instituto Federal da Paraiba, Joao Pessoa, 58800-970 PB, Brazil
DOI
https://doi.org/10.3390/proceedings1040536
Journal volume & issue
Vol. 1,
no. 4
Abstract
Read online
The detection of Leishmania spp. through a disposable surface plasmon resonance (SPR) biochip, using mono- and polychromatic sources, is presented here. A highly specific and sensitive-constrained synthetic peptide (LC2) derived from a mimotope of Leishmania chagasi antigen was immobilized on the sensing region using a simple protocol. ELISA- and PCR-confirmed negative and positive sera of Leishmania chagasi infected patients were used. Detection of positive IgG showed a fast response with good sensitivity and specificity for the proposed method.
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