Antioxidant and Biological Activity of Mexican Madroño Fruit (<i>Arbutus arizonica</i>)
Imelda N. Monroy-García,
Pilar Carranza-Rosales,
Irma Edith Carranza-Torres,
Lelie Denisse Castro-Ochoa,
Vianey González-Villasana,
Alma Rosa Islas-Rubio,
Ezequiel Viveros-Valdez
Affiliations
Imelda N. Monroy-García
Departamento de Ingeniería Química y Bioquímica, Tecnológico Nacional de México, Instituto Tecnológico de Los Mochis, Juan de Dios Bátiz y 20 de Noviembre, Los Mochis 81259, Sinaloa, Mexico
Pilar Carranza-Rosales
Centro de Investigación Biomédica de Noreste, Instituto Mexicano del Seguro Social, Calle Jesús Dionisio González #501, Monterrey 64720, Nuevo León, Mexico
Irma Edith Carranza-Torres
Centro de Investigación Biomédica de Noreste, Instituto Mexicano del Seguro Social, Calle Jesús Dionisio González #501, Monterrey 64720, Nuevo León, Mexico
Lelie Denisse Castro-Ochoa
Departamento de Ingeniería Química y Bioquímica, Tecnológico Nacional de México, Instituto Tecnológico de Los Mochis, Juan de Dios Bátiz y 20 de Noviembre, Los Mochis 81259, Sinaloa, Mexico
Vianey González-Villasana
Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Pedro de Alba S/N, San Nicolás de los Garza 66450, Nuevo León, Mexico
Alma Rosa Islas-Rubio
Coordinacion de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C. Gustavo Enrique Astiazaran Rosas #46, Hermosillo 83304, Sonora, Mexico
Ezequiel Viveros-Valdez
Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Pedro de Alba S/N, San Nicolás de los Garza 66450, Nuevo León, Mexico
The fruit of the Mexican madroño (Arbutus arizonica) has been consumed since pre-Columbian times by North American tribes and native groups in Mexico. Despite this, reports on its chemical composition and biological activity are limited. This work aims to determine the antioxidant, antiproliferative, and digestive enzyme inhibition activities of the methanol amberlite-retained extract of Mexican madroño. Results showed that madroño fruit is rich in antioxidants: DPPH (EC50 = 0.89 ± 0.03 mg/mL), TEAC (1078 ± 4.9 μM/g), and hemolysis inhibition (IC50 = 358.07 μg/mL), with high phenolic and flavonoid content at 15.92 ± 3.2 mg GAE/g and 4.33 ± 0.3 mg CA/g, respectively. Using analytical chromatography, gallic acid, vanillic acid, chlorogenic acid, ferulic acid, quercetin, and rutin were quantified. The extract also showed α-glucosidase inhibition (IC50 = 3.1 ± 0.17 mg/mL), but no inhibition against α-amylase and lipase (>5 mg/mL), while showing antiproliferative activity against HeLa, HT-29, and MCF-7 cancer cell lines. These results point towards an interesting potential for the fruit of the A. arizonica as chemopreventive and hold potential for elaborating functional foods.
