The effect of systemic melatonin administration on the intensity of free radical damage to lipids and proteins in the burn wound in the dynamics of experimental thermal injury

Abstract

Aim. To assess the effect of melatonin (MT) on the content of lipid peroxidation (LPO) and protein oxidation (PO) products in the tissue homogenate from the burn wound in experimental thermal injury (TI).Materials and methods. Third-degree (IIIA) TI with a relative area of 3.5% was modeled on male Wistar rats via contact of the skin with boiling water. Intraperitoneal administration of MT (10 mg / kg) was performed once a day for 5 days. On days 5, 10, and 20, LPO products in the heptane and isopropanol phases of lipid extraction and PO products were determined in the tissue homogenate from the burn wound.Results. The content of secondary and end products of LPO in the heptane phase and end products in the isopropanol phase increased in the wound. The content of primary and secondary PO products of neutral nature increased on days 5, 10, and 20, and the level of secondary PO products of neutral nature elevated on days 10 and 20. Administration of MT reduced the content of LPO end products in the heptane phase, secondary and end products of LPO in the isopropanol phase, and the total amount of PO products due to primary and secondary products of neutral nature.Conclusion. In the 20-day follow-up, LPO and PO products accumulated in the burn wound. The administration of MT at a total dose of 50 mg / kg led to reduction and partial restoration of the content of LPO and POM products, which can limit secondary alterations and accelerate healing of the burn wound.

Keywords

• мелатонин
• термическая травма
• перекисное окисление липидов
• окислительная модификация белков