Analysis of the Nebulin-Related Anchoring Protein Gene (N-RAP) SNP Polymorphism (C/T) in Slovak Warmblood Horse by PCR-RFLP Method

Abstract

The show-jumping is a important breeding goal in many breeds of warmblood horses. By using of genome-wide association studies (GWAS) were discovered a several genes which could be identified as candidate genes for physical performance in show jumping of horses. The one of this genes is the nebulin-related anchoring protein gene (NRAP) localized on ECA1. The aim of this work was genotyping the Slovak warmblood horse for SNP mutation (rs68545483) in NRAP gene and analysis of genotype structure in population of Slovak warmblood horses. The SNP polymorphism C/T of the N-RAP gene was studied in a group of 40 animals. Genomic DNA was isolated from samples of blood by using salting-out method. The SNP C/T was analyzed by PCR-RFLP method with restriction endonuclease BseGI. The allele T was detected by two restriction fragments 93 bp and 40 bp and the allele C with 133 bp fragment. In the analyzed population of Slovak warmblood horses were detected the following frequency of alleles and genotypes for the SNP polymorphisms C/T of the N-RAP gene. Frequencies of allele T and allele C were 0.6125 and 0.3875 and frequencies of genotypes were 0.15 (genotype CC), 0.475 (genotype CT) and 0.375 (genotype TT).

Keywords

• n-rap gene
• pcr-rflp
• show-jumping
• slovak warmblood horse