Neural Regeneration Research (Jan 2015)

Neurons in the hippocampal CA1 region, but not the dentate gyrus, are susceptible to oxidative stress in rats with streptozotocin-induced type 1 diabetes

  • Sang Gun Lee,
  • Dae Young Yoo,
  • Hyo Young Jung,
  • Sung Min Nam,
  • Jong Whi Kim,
  • Jung Hoon Choi,
  • Sun Shin Yi,
  • Moo-Ho Won,
  • Yeo Sung Yoon,
  • In Koo Hwang,
  • Seung Myung Moon

DOI
https://doi.org/10.4103/1673-5374.153695
Journal volume & issue
Vol. 10, no. 3
pp. 451 – 456

Abstract

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In this study, we investigated the effects of streptozotocin-induced type 1 diabetes on antioxidant-like protein-1 immunoreactivity, protein carbonyl levels, and malondialdehyde formation, a marker for lipid peroxidation, in the hippocampus. For this study, streptozotocin (75 mg/kg) was intraperitoneally injected into adult rats to induce type 1 diabetes. The three experimental parameters were determined at 2, 3, 4 weeks after streptozotocin treatment. Fasting blood glucose levels significantly increased by 20.7-21.9 mM after streptozotocin treatment. The number of antioxidant-like protein-1 immunoreactive neurons significantly decreased in the hippocampal CA1 region, but not the dentate gyrus, 3 weeks after streptozotocin treatment compared to the control group. Malondialdehyde and protein carbonyl levels, which are modified by oxidative stress, significantly increased with a peak at 3 weeks after malondialdehyde treatment, and then decreased 4 weeks after malondialdehyde treatment. These results suggest that neurons in the hippocampal CA1 region, but not the dentate gyrus, are susceptible to oxidative stress 3 weeks after malondialdehyde treatment.

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