LSM6 promotes cell proliferation and migration regulated by HMGB1 in laryngeal squamous cell carcinoma

Dengbin Ma; Jiayi Li; Hui Li; Yao Tang; Xia Gao; Hong Chen; Xiaoyun Qian; Xiaohui Shen

Engineered Regeneration (Jun 2024)

LSM6 promotes cell proliferation and migration regulated by HMGB1 in laryngeal squamous cell carcinoma

Dengbin Ma,
Jiayi Li,
Hui Li,
Yao Tang,
Xia Gao,
Hong Chen,
Xiaoyun Qian,
Xiaohui Shen

Affiliations

Dengbin Ma: Department of Otolaryngology Head and Neck Surgery, Research Institute of Otolaryngology, Nanjing Drum Tower Hospital, Jiangsu Provincial Key Medical Discipline, Affiliated Hospital of medical School, Nanjing University, Nanjing, 210008, China
Jiayi Li: Department of Otolaryngology Head and Neck Surgery, Research Institute of Otolaryngology, Nanjing Drum Tower Hospital, Jiangsu Provincial Key Medical Discipline, Affiliated Hospital of medical School, Nanjing University, Nanjing, 210008, China; Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, 321 Zhongshan Road, Nanjing, 210008, China
Hui Li: Department of Otolaryngology Head and Neck Surgery, Research Institute of Otolaryngology, Nanjing Drum Tower Hospital, Jiangsu Provincial Key Medical Discipline, Affiliated Hospital of medical School, Nanjing University, Nanjing, 210008, China
Yao Tang: Department of Otorhinolaryngology, Minda Hospital of Hubei Minzu University, Enshi, 445000, China
Xia Gao: Department of Otolaryngology Head and Neck Surgery, Research Institute of Otolaryngology, Nanjing Drum Tower Hospital, Jiangsu Provincial Key Medical Discipline, Affiliated Hospital of medical School, Nanjing University, Nanjing, 210008, China
Hong Chen: Department of Otolaryngology Head and Neck Surgery, Research Institute of Otolaryngology, Nanjing Drum Tower Hospital, Jiangsu Provincial Key Medical Discipline, Affiliated Hospital of medical School, Nanjing University, Nanjing, 210008, China; Corresponding authors.
Xiaoyun Qian: Department of Otolaryngology Head and Neck Surgery, Research Institute of Otolaryngology, Nanjing Drum Tower Hospital, Jiangsu Provincial Key Medical Discipline, Affiliated Hospital of medical School, Nanjing University, Nanjing, 210008, China; Corresponding authors.
Xiaohui Shen: Department of Otolaryngology Head and Neck Surgery, Research Institute of Otolaryngology, Nanjing Drum Tower Hospital, Jiangsu Provincial Key Medical Discipline, Affiliated Hospital of medical School, Nanjing University, Nanjing, 210008, China; Corresponding authors.

Journal volume & issue: Vol. 5, no. 2
pp. 247 – 254

Abstract

Read online

Elevated levels of high mobility group protein B1 (HMGB1) play a significant role in the pathogenesis of many diseases, but is particularly important for the formation of malignant tumors. Nonetheless, the function of HMGB1 and the underlying mechanism of laryngeal squamous cell carcinoma (LSCC) remain incompletely understood, causing uncertainty. Here we found immunohistochemistry from 97 LSCC tissues showed HMGB1 was upregulated, which was associated with poor differentiation. HMGB1 knockdown could significantly inhibit wound closure and colony formation. The full-genome gene expression microarray was performed to investigate the mechanism. After knockdown of HMGB1 by siRNA, among the expressed differential genes, 10 genes were randomly selected for validation. Then, shRNA lentivirus targeting these genes were constructed to explore their role in LSCC by cell proliferation assay. LSM6 downregulation was dramatically promoted by HMGB1 knockdown, resulting in higher expression in LSCC tissues. Furthermore, downregulation of LSM6 could significantly suppress cell proliferation, migration and colony formation. This study indicated that HMGB1 promoted LSCC cell malignant phenotypes through regulation of LSM6. We anticipate that HMGB1-LSM6 could be a putative therapeutic target for LSCC.

Published in Engineered Regeneration

ISSN: 2666-1381 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Science: Biology (General): Life
Website: https://www.keaipublishing.com/en/journals/engineered-regeneration/

About the journal

Abstract

Keywords