A draft map of the mouse pluripotent stem cell spatial proteome

Andy Christoforou; Claire M. Mulvey; Lisa M. Breckels; Aikaterini Geladaki; Tracey Hurrell; Penelope C. Hayward; Thomas Naake; Laurent Gatto; Rosa Viner; Alfonso Martinez Arias; Kathryn S. Lilley

doi:10.1038/ncomms9992

Nature Communications (Jan 2016)

A draft map of the mouse pluripotent stem cell spatial proteome

Andy Christoforou,
Claire M. Mulvey,
Lisa M. Breckels,
Aikaterini Geladaki,
Tracey Hurrell,
Penelope C. Hayward,
Thomas Naake,
Laurent Gatto,
Rosa Viner,
Alfonso Martinez Arias,
Kathryn S. Lilley

Affiliations

Andy Christoforou: Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge
Claire M. Mulvey: Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge
Lisa M. Breckels: Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge
Aikaterini Geladaki: Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge
Tracey Hurrell: Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge
Penelope C. Hayward: Department of Genetics, University of Cambridge
Thomas Naake: Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge
Laurent Gatto: Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge
Rosa Viner: Thermo Fisher Scientific
Alfonso Martinez Arias: Department of Genetics, University of Cambridge
Kathryn S. Lilley: Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge

DOI: https://doi.org/10.1038/ncomms9992
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

Read online

The spatial location of proteins within a cell is a key element of protein function. Here the authors describe hyperLOPIT—a proteomics workflow that allows the simultaneous assignment of thousands of proteins to subcellular niches with high resolution—and apply it to mouse pluripotent stem cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal