Adeno-associated viral vector gene expression in the adult rat spinal cord following remote vector delivery

Nicholas M Boulis; Aaron J Noordmans; Debbie K Song; Michael J Imperiale; Adam Rubin; Paola Leone; Matthew During; Eva L Feldman

Neurobiology of Disease (Dec 2003)

Adeno-associated viral vector gene expression in the adult rat spinal cord following remote vector delivery

Nicholas M Boulis,
Aaron J Noordmans,
Debbie K Song,
Michael J Imperiale,
Adam Rubin,
Paola Leone,
Matthew During,
Eva L Feldman

Affiliations

Nicholas M Boulis: Neurosurgery, Cleveland Clinic Foundation, Cleveland, OH, USA; Neurology, University of Michigan, Ann Arbor, MI, USA; Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA; Otolaryngology, University of Michigan, Ann Arbor, MI, USA; Cell and Gene Therapy Center, University of Medicine and Dentistry of New Jersey, Camden, NJ, USA; Center for Gene Therapy, University of Michigan, Ann Arbor, MI, USA; Functional Genomics and Translational Neuroscience Laboratory, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand
Aaron J Noordmans: Neurosurgery, Cleveland Clinic Foundation, Cleveland, OH, USA; Neurology, University of Michigan, Ann Arbor, MI, USA; Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA; Otolaryngology, University of Michigan, Ann Arbor, MI, USA; Cell and Gene Therapy Center, University of Medicine and Dentistry of New Jersey, Camden, NJ, USA; Center for Gene Therapy, University of Michigan, Ann Arbor, MI, USA; Functional Genomics and Translational Neuroscience Laboratory, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand
Debbie K Song: Neurosurgery, Cleveland Clinic Foundation, Cleveland, OH, USA; Neurology, University of Michigan, Ann Arbor, MI, USA; Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA; Otolaryngology, University of Michigan, Ann Arbor, MI, USA; Cell and Gene Therapy Center, University of Medicine and Dentistry of New Jersey, Camden, NJ, USA; Center for Gene Therapy, University of Michigan, Ann Arbor, MI, USA; Functional Genomics and Translational Neuroscience Laboratory, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand
Michael J Imperiale: Neurosurgery, Cleveland Clinic Foundation, Cleveland, OH, USA; Neurology, University of Michigan, Ann Arbor, MI, USA; Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA; Otolaryngology, University of Michigan, Ann Arbor, MI, USA; Cell and Gene Therapy Center, University of Medicine and Dentistry of New Jersey, Camden, NJ, USA; Center for Gene Therapy, University of Michigan, Ann Arbor, MI, USA; Functional Genomics and Translational Neuroscience Laboratory, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand
Adam Rubin: Neurosurgery, Cleveland Clinic Foundation, Cleveland, OH, USA; Neurology, University of Michigan, Ann Arbor, MI, USA; Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA; Otolaryngology, University of Michigan, Ann Arbor, MI, USA; Cell and Gene Therapy Center, University of Medicine and Dentistry of New Jersey, Camden, NJ, USA; Center for Gene Therapy, University of Michigan, Ann Arbor, MI, USA; Functional Genomics and Translational Neuroscience Laboratory, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand
Paola Leone: Neurosurgery, Cleveland Clinic Foundation, Cleveland, OH, USA; Neurology, University of Michigan, Ann Arbor, MI, USA; Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA; Otolaryngology, University of Michigan, Ann Arbor, MI, USA; Cell and Gene Therapy Center, University of Medicine and Dentistry of New Jersey, Camden, NJ, USA; Center for Gene Therapy, University of Michigan, Ann Arbor, MI, USA; Functional Genomics and Translational Neuroscience Laboratory, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand
Matthew During: Neurosurgery, Cleveland Clinic Foundation, Cleveland, OH, USA; Neurology, University of Michigan, Ann Arbor, MI, USA; Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA; Otolaryngology, University of Michigan, Ann Arbor, MI, USA; Cell and Gene Therapy Center, University of Medicine and Dentistry of New Jersey, Camden, NJ, USA; Center for Gene Therapy, University of Michigan, Ann Arbor, MI, USA; Functional Genomics and Translational Neuroscience Laboratory, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand
Eva L Feldman: Neurosurgery, Cleveland Clinic Foundation, Cleveland, OH, USA; Neurology, University of Michigan, Ann Arbor, MI, USA; Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA; Otolaryngology, University of Michigan, Ann Arbor, MI, USA; Cell and Gene Therapy Center, University of Medicine and Dentistry of New Jersey, Camden, NJ, USA; Center for Gene Therapy, University of Michigan, Ann Arbor, MI, USA; Functional Genomics and Translational Neuroscience Laboratory, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand

Journal volume & issue: Vol. 14, no. 3
pp. 535 – 541

Abstract

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The current investigation tests whether adeno-associated viral vectors (rAAV) undergo remote delivery to the spinal cord via peripheral nerve injection as previously demonstrated with adenoviral vectors. The sciatic nerves of adult rats (n = 10) were injected with either an rAAV (rAAVCMV-lacZ) or adenoviral (AdCMV-lacZ) vector (1.4 × 107 particles/ml). After 21 days, the rAAV group demonstrated significantly higher spinal cord viral expression than the adenoviral group (P < 0.024). A second group of rats was injected with rAAV expressing the green fluorescence protein (GFP) reporter gene. GFP was detected 21 days after unilateral sciatic nerve injection in the neurons of the dorsal root ganglion and spinal cord. The codistribution of the viral genome and transgene in CNS neurons was confirmed with in situ hybridization. In summary, rAAV genes are expressed in CNS neurons following peripheral nerve injection at levels exceeding those seen following remote adenovirus injection.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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