Kinetic compartmentalization by unnatural reaction for itaconate production

Dae-yeol Ye; Myung Hyun Noh; Jo Hyun Moon; Alfonsina Milito; Minsun Kim; Jeong Wook Lee; Jae-Seong Yang; Gyoo Yeol Jung

doi:10.1038/s41467-022-33033-1

Nature Communications (Sep 2022)

Kinetic compartmentalization by unnatural reaction for itaconate production

Dae-yeol Ye,
Myung Hyun Noh,
Jo Hyun Moon,
Alfonsina Milito,
Minsun Kim,
Jeong Wook Lee,
Jae-Seong Yang,
Gyoo Yeol Jung

Affiliations

Dae-yeol Ye: Department of Chemical Engineering, Pohang University of Science and Technology
Myung Hyun Noh: Department of Chemical Engineering, Pohang University of Science and Technology
Jo Hyun Moon: Department of Chemical Engineering, Pohang University of Science and Technology
Alfonsina Milito: Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB
Minsun Kim: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology
Jeong Wook Lee: Department of Chemical Engineering, Pohang University of Science and Technology
Jae-Seong Yang: Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB
Gyoo Yeol Jung: Department of Chemical Engineering, Pohang University of Science and Technology

DOI: https://doi.org/10.1038/s41467-022-33033-1
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

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Unlike eukaryotic system, bacterial hosts lack membranous system, which is one of the limitations for efficient metabolic engineering. Here, the authors report a kinetic compartmentalization strategy to increase substrate availability from competitive reactions for the efficient production of itaconate in E. coli.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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