Effects of Storage Time on Electrolyte Content and pH Value in Rat Serum Samples

Abstract

Objective To observe the effects of serum sample placement time on the detection results of K +, Na+, Ca2+, Cl - and pH in sera of SD rats. MethodsThe sera of 20 male and 20 female SD rats were separated after blood collection from abdominal vein. The samples were covered and stored in a refrigerator at 2-8 ℃. The electrolyte indexes including K+, Na+, Ca2+, Cl- and pH in serum were measured at 7 time points of 0 h, 2 h, 4 h, 6 h, 24 h, 48 h and 120 h after storage. Results There were no significant differences in the detection results of K+ and Na+ at each time point (P > 0.05). Compared with the test results at 0 h, the content of Cl- increased significantly at 48 h and 120 h (P 0.05). Conclusion The storage time of serum samples of SD rats has a significant impact on the detection results of Ca2+, Cl- and pH in serum. The samples should be covered and stored in a refrigerator at 2-8 ℃ and completed within 6 h after sample preparation.

Keywords

• serum
• placement time
• k+
• na+
• ca2+
• ph
• sprague-dawley rats