Evaluation of polymer viability of Crossandra infundibuliformis L. (Acanthaceae) based on colorimetric tests of 2,3,5-trifenyletrazole (TTC) and lugol 2%

Abstract

Crossandra infundibuliformis L. (Acanthaceae) is a shrub native to India. It is an important ornamental plant in horticulture, possessing medicinal value, used to treat various diseases, in the extract of its leaves contains properties with antifungal, antimicrobial, etc. activities. The objective of this work was to estimate the pollen viability of C. infundibuliformis, found in three sites in the municipality of Alta Floresta-MT. For the study, a solution of 2,3,5-triphenyltetrazolium (TTC) at concentrations of 0.075% and 0.30% was used with the material exposed at four different times and Lugol 2% dye. In the preparation of the slides using TTC, one drop of each treatment on the slide was then covered with a cover slip. In the Lugol 2% test, the anthers crushing technique was used in the preparation of the slides. In the estimation of the pollen viability of populations 01, 02 and 03 of C. infundibuliformis, colorimetric tests (TTC) and Lugol 2%) were efficient in distinguishing viable pollens from non-viable pollens. It was observed that for the two concentrations there was a gradual increase of the means as the time of exposure increased. The concentration of 0.30% presented the highest averages of pollen viability. The concentration of 0.075% presented average percentages of viability at all times. Before the treatments to estimate the pollen viability of the species, the concentration of 0.30% in the time of exposure of 24 hours was the best treatment for the distinct differentiation of the pollen grains. The viability averages of C. infundibuliformis were averages for TTC concentrations and high for Lugol dye 2%.

Keywords

• ornamental plant, estimate, dye