Aedes (Ochlerotatus) lepidus (Cerqueira & Paraense, 1945) is reported from a cave in Templo dos Pilares Municipal Park, Mato Grosso do Sul state, Brazil. A single exploratory collection of culicids was carried out using CDC light traps on 16 November 2015. Four larvae and 11 adults (1♂, 10♀) were collected. The larvae were foundin a water-filled hole in a stone. This is the first record of the species in Mato Grosso do Sul, which increases to 63 the number of culicids species recorded in the state.
