Protocol for efficient micropropagation of spring gentian and sand jurinea

Abstract

Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg.l-1of glutamine, 200 mg.l-1 of casein hydrolysate, 30 g.l-1 of sucrose, 0.2 mg.l-1 of BAP, and 0.1 mg.l-1 of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg.l-1 of IBA 70-75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J. cyanoides.

Keywords

• gentiana verna
• jurinea cyanoides
• in vitro reproduction
• organogenesis