Improved procedure for the extraction of lipids from human erythrocytes

Abstract

A procedure for the extraction of human erythrocyte lipids using chloroform–isopropanol 7:11 (v/v) is described. It is simple and reproducible, affords almost quantitative extraction of cholesterol and phospholipid, uses a single extraction tube, and yields an extract only slightly contaminated by heme.

Keywords

• human
• erythrocyte
• lipid
• extraction
• chloroform-isopropanol
• Folch