Multi-Omics Revealed Peanut Root Metabolism Regulated by Exogenous Calcium under Salt Stress
Xuan Dong,
Yan Gao,
Xuefeng Bao,
Rongjin Wang,
Xinyu Ma,
Hui Zhang,
Yifei Liu,
Lanshu Jin,
Guolin Lin
Affiliations
Xuan Dong
College of Land and Environment, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Yan Gao
College of Land and Environment, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Xuefeng Bao
College of Land and Environment, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Rongjin Wang
College of Land and Environment, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Xinyu Ma
Testing Center for Agricultural Product Safety and Environmental Quality, Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, No. 72, Culture Road, Shenhe District, Shenyang 110017, China
Hui Zhang
College of Land and Environment, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Yifei Liu
College of Land and Environment, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Lanshu Jin
College of Land and Environment, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Guolin Lin
College of Land and Environment, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
High salinity severely inhibits plant seedling root development and metabolism. Although plant salt tolerance can be improved by exogenous calcium supplementation, the metabolism molecular mechanisms involved remain unclear. In this study, we integrated three types of omics data (transcriptome, metabolome, and phytohormone absolute quantification) to analyze the metabolic profiles of peanut seedling roots as regulated by exogenous calcium under salt stress. (1) exogenous calcium supplementation enhanced the allocation of carbohydrates to the TCA cycle and plant cell wall biosynthesis rather than the shikimate pathway influenced by up-regulating the gene expression of antioxidant enzymes under salt stress; (2) exogenous calcium induced further ABA accumulation under salt stress by up-regulating the gene expression of ABA biosynthesis key enzymes AAO2 and AAO3 while down-regulating ABA glycosylation enzyme UGT71C5 expression; (3) exogenous calcium supplementation under salt stress restored the trans-zeatin absolute content to unstressed levels while inhibiting the root cis-zeatin biosynthesis.
