Assessment of Growth-Related Parameters, Immune-Biochemical Profile, and Expression of Selected Genes of Red Tilapia Fed with Roselle Calyces (<i>Hibiscus sabdariffa</i>) Extract
Amany M. Diab,
Eslam E. Eldeghaidy,
Mohamed H. Abo-Raya,
Mustafa Shukry,
Ahmed Abdeen,
Samah F. Ibrahim,
Liana Fericean,
Mohamed Abdo,
Malik M. Khalafalla
Affiliations
Amany M. Diab
Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Eslam E. Eldeghaidy
Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Mohamed H. Abo-Raya
Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Mustafa Shukry
Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Ahmed Abdeen
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt
Samah F. Ibrahim
Department of Clinical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Liana Fericean
Department of Biology and Plant Protection, Faculty of Agriculture, University of Life Sciences “King Michael I” from Timișoara, 300645 Timisoara, Romania
Mohamed Abdo
Department of Animal Histology and Anatomy, School of Veterinary Medicine, Badr University in Cairo (BUC), Badr City 32897, Egypt
Malik M. Khalafalla
Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
The purpose of this research was to determine whether or not supplementing a diet with ethanolic roselle calyces extract (ER) had any effect on the rate of growth, intestinal morphometry, total carotene in skin and muscle, blood profile, immunity status, and the expression response of red tilapia. The ER was added to four experimental diets at 0% (0 g kg−1), 0.5% (5 g kg−1), 1% (10 g kg−1), and 2% (20 g kg−1), which were designated as ER0 (control group), ER0.5, ER1, and ER2, respectively. The results show that ER1 induced higher weights (final weight, weight gain, specific growth rate, and weight gain rate) and all ER groups had considerably (p p −1 ER. In addition, diets supplemented with different levels of ER stimulated phagocytic activity (p IGF-1, GHr, SOD, TNF-α, and LPL, whereas MSTN, HSP 70, and FAS were downregulated. In conclusion, dietary ER supplementations are advantageous for red tilapia because they improve immunological and growth-related parameters.
