Efficacy of goldenberry in improving obesity-induced hemoglobin conformational structure changes in wistar rats: A biophysical perspective
Sherif A. Abdelmottaleb Moussa,
Samir W. Aziz,
Samir A. Bashandy,
Marawan Abd elbaset,
Noha A. Abd El-Latif,
Sherif M. Afifi,
Tuba Esatbeyoglu,
Sayed A. El Toumy,
Josline Y. Salib
Affiliations
Sherif A. Abdelmottaleb Moussa
Biophysics Laboratory, Biochemistry Department, National Research Centre, P.O. 12622, Cairo, Egypt; Corresponding author. Department of Biochemistry, National Research Centre, Cairo, Egypt.
Samir W. Aziz
Biophysics Laboratory, Biochemistry Department, National Research Centre, P.O. 12622, Cairo, Egypt
Samir A. Bashandy
Pharmacology Department, National Research Centre, P.O. 12622, Cairo, Egypt
Marawan Abd elbaset
Pharmacology Department, National Research Centre, P.O. 12622, Cairo, Egypt
Noha A. Abd El-Latif
Biophysics Laboratory, Biochemistry Department, National Research Centre, P.O. 12622, Cairo, Egypt
Sherif M. Afifi
Department for Life Quality Studies, Rimini Campus, University of Bologna, Corso d’Augusto 237, 47921, Rimini, Italy
Tuba Esatbeyoglu
Department of Molecular Food Chemistry and Food Development, Institute of Food and One Health, Gottfried Wilhelm Leibniz University Hannover, Am Kleinen Felde 30, 30167, Hannover, Germany; Corresponding author. Department of Food Development and Food Quality, Gottfried Wilhelm Leibniz University Hannover, Germany.
Sayed A. El Toumy
Chemistry of Tannins Department, National Research Centre, P.O. 12622, Cairo, Egypt
Josline Y. Salib
Chemistry of Tannins Department, National Research Centre, P.O. 12622, Cairo, Egypt
The relationship between obesity and the conformational structure of hemoglobin (Hb) has not been extensively investigated. This study aims to elucidate the dielectric parameters that distinguish the Hb molecule under obese conditions and following treatment with goldenberry (GB) extract, compared to a control group. The dielectric parameters analyzed include the loss factor (D), relaxation time (τ), dielectric increment (Δε), relative permittivity (έ), dielectric loss (ε''), conductivity (σ), and Cole-Cole parameters (α), measured across a frequency range of 20 Hz to 3 MHz. Significant differences in dielectric parameters were observed between obese rats and those treated with GB extract. Obese rats exhibited higher dielectric values compared to the control group, while rats treated with low and high doses of GB extract showed marked changes in Hb conformational structure. This study highlights the potential of dielectric parameters as biophysical markers for detecting hemoglobin conformational changes. Furthermore, it suggests that dielectric behavior could serve as an early indicator for assessing the severity of obesity and its related complications.
