Induction of Drug-Resistance and Production of a Culture Medium Able to Induce Drug-Resistance in Vinblastine Untreated Murine Myeloma Cells
Valentina Laghezza Masci,
Davide Stefanoni,
Angelo D’Alessandro,
Marta Zambelli,
Lorenzo Modesti,
Daniele Pollini,
Elisa Ovidi,
Antonio Tiezzi
Affiliations
Valentina Laghezza Masci
Laboratory of Plant Cytology and Biotechnology of Natural Compounds, Department for the Innovation in Biological, Agri-Food and Forestal Systems, University of Tuscia, Largo dell’Università, snc, 01100 Viterbo, Italy
Davide Stefanoni
Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA
Angelo D’Alessandro
Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA
Marta Zambelli
Laboratory of Plant Cytology and Biotechnology of Natural Compounds, Department for the Innovation in Biological, Agri-Food and Forestal Systems, University of Tuscia, Largo dell’Università, snc, 01100 Viterbo, Italy
Lorenzo Modesti
Laboratory of Plant Cytology and Biotechnology of Natural Compounds, Department for the Innovation in Biological, Agri-Food and Forestal Systems, University of Tuscia, Largo dell’Università, snc, 01100 Viterbo, Italy
Daniele Pollini
Laboratory of Plant Cytology and Biotechnology of Natural Compounds, Department for the Innovation in Biological, Agri-Food and Forestal Systems, University of Tuscia, Largo dell’Università, snc, 01100 Viterbo, Italy
Elisa Ovidi
Laboratory of Plant Cytology and Biotechnology of Natural Compounds, Department for the Innovation in Biological, Agri-Food and Forestal Systems, University of Tuscia, Largo dell’Università, snc, 01100 Viterbo, Italy
Antonio Tiezzi
Laboratory of Plant Cytology and Biotechnology of Natural Compounds, Department for the Innovation in Biological, Agri-Food and Forestal Systems, University of Tuscia, Largo dell’Università, snc, 01100 Viterbo, Italy
Cancer therapies use different compounds of synthetic and natural origin. However, despite some positive results, relapses are common, as standard chemotherapy regimens are not fully capable of completely eradicating cancer stem cells. While vinblastine is a common chemotherapeutic agent in the treatment of blood cancers, the development of vinblastine resistance is often observed. Here, we performed cell biology and metabolomics studies to investigate the mechanisms of vinblastine resistance in P3X63Ag8.653 murine myeloma cells. Treatment with low doses of vinblastine in cell media led to the selection of vinblastine-resistant cells and the acquisition of such resistance in previously untreated, murine myeloma cells in culture. To determine the mechanistic basis of this observation, we performed metabolomic analyses of resistant cells and resistant drug-induced cells in a steady state, or incubation with stable isotope-labeled tracers, namely, 13C 15N-amino acids. Taken together, these results indicate that altered amino acid uptake and metabolism could contribute to the acquisition of vinblastine resistance in blood cancer cells. These results will be useful for further research on human cell models.
