Applications of combinations and concentrations of different phytohormones for the induction of callus, shoot, and root of cannabis sativaunder tissue culture technique

Abstract

Cannabis sativa is a significant crop of the family (cannabaceae).Interest has been resurgent in the usage of the cannabis plant for medical purposes, industrial fibre, seed oil, food, and recreation.Auxin and cytokinins act antagonistically with each other in which cytokinins promote shoot branching and inhibit root branching, while auxin inhibits shoot branching and promotes root branching. Young cannabis leaves and petioles were used and inoculated in the MS medium with various combinations. Murashige and Skoog (MS) medium for explant were developed in additions of 25µl-450µl 2, 4 D, 25µl-450µl Kinetin, and 50µl-450µl BAP for callus induction. Shoot induction was developed on 2-3ml/l BAP-supplemented MS media from the callus. Root induction was developed from the shoot on MS5ml/2 litters (indole-3-acetic acid) IAA + 8ml/2 litters (indole-3-butyric acid)IBA-supplemented media.Seeds of cannabis were inoculated in the MS medium. After one week, seed germination was observed in the seeds of the DG Khan variety of Cannabis. MS media found better and more abundant Callus induction with 450µl 2, 4D +450µl Kinetin+450µlBAP from cannabis sativa leaves. 3ml/l BAP observed the high growth of the shoot. Better growth of roots was observed by 5ml/2 litters (indole-3-acetic acid) IAA + 8ml/2 litters (indole-3-butyric acid) IBA. In the present study, seed, callus, shoot, root, and meristem growth were observed using the combination and concentration of different growth regulators. By using different phytohormones, the growth rate and productivity of Cannabiswere significantly increased, which can be valuable for medical purposes.

Keywords

• Cannabis sativa; Murashige and Skoog; indole-3-acetic acid; Kinetin 2,4D; BAP; Kinetin;IAA; IBA.