High-resolution ex vivo NMR spectroscopy of human Z α1-antitrypsin

Alistair M. Jagger; Christopher A. Waudby; James A. Irving; John Christodoulou; David A. Lomas

doi:10.1038/s41467-020-20147-7

Nature Communications (Dec 2020)

High-resolution ex vivo NMR spectroscopy of human Z α1-antitrypsin

Alistair M. Jagger,
Christopher A. Waudby,
James A. Irving,
John Christodoulou,
David A. Lomas

Affiliations

Alistair M. Jagger: UCL Respiratory, Rayne Institute, University College London
Christopher A. Waudby: Institute of Structural and Molecular Biology, University College London and School of Crystallography, Birkbeck College, University of London
James A. Irving: UCL Respiratory, Rayne Institute, University College London
John Christodoulou: Institute of Structural and Molecular Biology, University College London and School of Crystallography, Birkbeck College, University of London
David A. Lomas: UCL Respiratory, Rayne Institute, University College London

DOI: https://doi.org/10.1038/s41467-020-20147-7
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

Read online

α1-Antitrypsin (AAT) is a 52 kDa serum glycoprotein, the misfolding and polymerisation of which is associated with COPD and liver disease. Here the authors demonstrate the use of high-resolution multidimensional solution-state NMR spectroscopy to characterise the structure and dynamics in solution of Z AAT purified directly from clinical patients.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal