Determination of Biogenic Amines in Different Parts of <i>Lycium barbarum</i> L. by HPLC with Precolumn Dansylation
Yun Ai,
Yan Ni Sun,
Li Liu,
Fang Yuan Yao,
Yan Zhang,
Feng Yi Guo,
Wen Jie Zhao,
Jian Li Liu,
Ning Zhang
Affiliations
Yun Ai
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Sience, Northwest University, Xi’an 710069, China
Yan Ni Sun
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Sience, Northwest University, Xi’an 710069, China
Li Liu
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Sience, Northwest University, Xi’an 710069, China
Fang Yuan Yao
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Sience, Northwest University, Xi’an 710069, China
Yan Zhang
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Sience, Northwest University, Xi’an 710069, China
Feng Yi Guo
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Sience, Northwest University, Xi’an 710069, China
Wen Jie Zhao
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Sience, Northwest University, Xi’an 710069, China
Jian Li Liu
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Sience, Northwest University, Xi’an 710069, China
Ning Zhang
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Sience, Northwest University, Xi’an 710069, China
The aim of this work was to characterize biogenic amines (BAs) in different parts of Lycium barbarum L. using HPLC with dansyl chloride derivatization, and jointly, to provide referential data for further exploration and utilization of Lycium barbarum L. The linear correlation coefficients for all BAs were above 0.9989. The limits of detection and quantification were 0.015–0.075 and 0.05–0.25 μg/mL, respectively. The relative standard deviations for the intra-day and inter-day precision were 0.66–2.69% and 0.91–4.38%. The described method has good repeatability and intermediate precision for the quantitative determination of BAs in different parts of Lycium barbarum L. Satisfactory recovery for all amines was obtained (79.3–110.3%). The result showed that there were four kinds of BAs. The highest putrescine content (20.9 ± 3.2 mg/kg) was found in the flower. The highest histamine content (102.7 ± 5.8 mg/kg) was detected in the bark, and the highest spermidine (13.3 ± 1.6 mg/kg) and spermine (23.7 ± 2.0 mg/kg) contents were detected in the young leaves. The high histamine (HIS) content in the bark may be one of the reasons why all of the parts of Lycium barbarum L., except the bark, are used for medicine or food in China. Meanwhile, the issue of the high concentration of HIS should be considered when exploiting or utilizing the bark of Lycium barbarum L.
