Time-of-addition and Temperature-shift Assays to Determine Particular Step(s) in the Viral Life Cycle that is Blocked by Antiviral Substance(s)
Chie Aoki-Utsubo,
Ming Chen,
Hak Hotta
Affiliations
Chie Aoki-Utsubo
Department of International Health, Graduate School of Health Sciences, Kobe University, Kobe, Japan
Ming Chen
Department of Vaccine and Drug Development, Graduate School of Health Sciences, Kobe University, Kobe, Japan
Hak Hotta
Department of International Health, Graduate School of Health Sciences, Kobe University, Kobe, JapanDepartment of Vaccine and Drug Development, Graduate School of Health Sciences, Kobe University, Kobe, Japan, Faculty of Clinical Nutrition and Dietetics, Konan-Women’s University, Kobe, Japan
Viruses infect their host cells to produce progeny virus particles through the sequential steps of the viral life cycle, such as viral attachment, entry, penetration and post-entry events. This protocol describes time-of-addition and temperature-shift assays that are employed to explore which step(s) in the viral life cycle is blocked by an antiviral substance(s).
